منابع مشابه
Role of endoplasmic reticulum in biosynthesis of oat globulin precursors.
Oat (Avena sativa L.) groats were labeled with radioactive leucine and salt-soluble proteins were extracted and analyzed. Polyacrylamide gel electrophoresis followed by fluorography indicated two radioactive polypeptides with molecular weight 58 to 62 kilodaltons which were similar in size to unreduced globulin alpha-beta dimers. The role of endoplasmic reticulum in the synthesis of these globu...
متن کاملAntioxidant response and related gene expression in aged oat seed
To evaluate deterioration of oat seeds during storage, we analyzed oxygen radicals, antioxidant enzyme activity, proline content, and gene transcript levels in oat seeds with different moisture contents (MCs; 4, 16, and 28% w/w) during storage for 0, 6, and 12 months (CK, LT-6, and LT-12 treatments, respectively) at 4°C. The germination percentage decreased significantly with higher seed MCs an...
متن کاملMolecular Characterization of Oat Seed Globulins 1 Received for
We have isolated full-length cDNA clones that encode oat (Avena sativa) seed storage globulin mRNAs from a cDNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an acidic polypeptide of 293 amino acids and a basic polypeptide of 201 amino aci...
متن کاملLack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice
The major seed storage proteins (SSPs) in rice seeds have been classified into three types, glutelins, prolamins, and globulin, and the proportion of each SSP varies. It has been shown in rice mutants that when either glutelins or prolamins are defective, the expression of another type of SSP is promoted to counterbalance the deficit. However, we observed reduced abundances of glutelins and pro...
متن کاملCell-free Synthesis of Globulin by Developing Oat (Avena sativa L.) Seeds.
The primary storage protein synthesized during oat (Avena sativa L.) groat development is a globulin. Polysomes were isolated from oat groats 12 days after anthesis. These polysomes directed the incorporation of radioactive amino acids into protein in a cell-free protein synthesis system containing wheat germ supernatant. The Mg(2+) optimum was 4 mm, the pH optimum was 6-8, and the amount of am...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1983
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.72.1.161